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1.
Acta biol. colomb ; 23(1): 66-72, Jan.-Apr. 2018. tab, graf
Article in Spanish | LILACS | ID: biblio-886085

ABSTRACT

RESUMEN Se describe la anatomía foliar de las especies Gaiadendron punctatum y Tripodanthus belmirensis, al objeto de estudiar posibles caracteres que permitan una identificación precisa de estos dos géneros de la familia Loranthaceae, de hábito arbustivo o arbóreo. Las muestras se procesaron y sometieron a tinción con técnicas clásicas para su observación al microscopio óptico. Ambas especies presentaron similitudes como una epidermis monoestratificada y estomas de tipo rubiáceo. Sin embargo, se observó una composición anatómica claramente diferenciada en aspectos como el mesófilo, forma y ubicación de las células epidérmicas y la presencia de acumulaciones de súber en G. punctatum o idioblastos abundantes en T. belmirensis. Se construyó una clave dicotómica para la determinación de especies con base en caracteres anatómicos de la hoja entre las especies del género Tripodanthus y G. punctatum, además se discute brevemente el uso de caracteres anatómicos en la determinación y soporte de entidades taxonómicas diferenciables dentro de la familia Loranthaceae.


ABSTRACT Leaf anatomy of Gaiadendron punctatum and Tripodanthus belmirensis species is described with the aim of exploring possible characters that allow a precise identification of these two genera, characterized by tree or shrub habit, belonging to Loranthaceae family. Samples were processed and stained with routine techniques for observation on optical microscope. Both species showed similarities, such as one-layered epidermis and rubiaceous type stomata. However, a different anatomic composition was observed in aspects such as: mesophyll, position and shape of epidermic cells and presence of suber accumulations in G. punctatum, or numerous idioblasts in T. belmirensis. A dichotomous key was constructed for species determination based on anatomical leaf characters, between the species of genus Tripodanthus and G. punctatum. Furthermore, the use of anatomical characters in determination and support of distinguishable taxonomical entities inside Loranthaceae is also briefly discussed.

2.
Chinese Journal of Analytical Chemistry ; (12): 1683-1686, 2009.
Article in Chinese | WPRIM | ID: wpr-405434

ABSTRACT

A method was developed for the determination of zeranols (α,β-zearalanol,α,β-zearalenol,zear-alanone,zearalenone) with RRLC-MS/MS in the plant tissue. The samples were extracted with acetonitrile and reextracted with aqueous alkaline solution,cleaned up with MAX column and then determined by RRLC-MS/MS using multiple reaction monitoring ( MRM) scan mode. The results showed that the working curves were linear in the range of 0 -20 μg/kg. The limits of detection ( LOD) of zeranols were 0.5 μg/kg and the limits of quantification (LOQ) was 1. 0μg/kg. Extraction recoveries for zeranols ranged from 75. 8% to 105.4% and RSD was between 2.4% and 12.1%. The method is suitable for the determination of zeranols in the plant tissue.

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